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9339 - Research Technician I

This position will support studies focused on improving osteoporosis therapies particularly the efficacy of parathyroid hormone (PTH). This person will assist in maintaining a small experimental mouse colony by keeping breeding records, genotyping the pups, and by injecting the experimental adult mice with therapeutic agents. This technician will perform routine tissue harvest from the euthanized experimental mice. Routine cell culture maintenance will be part of the duties of the position. The person will be responsible for ordering lab supplies and preparing standard lab solutions. Some routine PCR and Western analyses of experimental samples will be required.

Primary Responsibilities Include:

• Maintain mouse colony: keep records of mice/breeding pairs and genotype pups; inject mice with treatment therapies • Maintain cell cultures: isolate, establish, feed, pass, and freeze cells for stocks from experimental mice; treat cells with therapeutic reagents for the study of experimental treatments • Harvest tissue/cell samples for analysis: euthanize mice and harvest bones and bone marrow cells for analysis. Deliver samples to appropriate lab/core facility for analyses. • Preparation of lab solutions: make buffers, fixatives and various solutions needed in running assays for cell culture or tissue processing. • Order lab supplies: maintain laboratory reagents and supplies via ordering from vendors. • Performance of PCR and Western analyses: these simple techniques are used to genotype mice from colony and to evaluate gene expression of experimental cell or bone extracts

Required: Bachelor's degree in science from an accredited institution; or advanced level science plus 2 years research experience; or high school or equivalent plus 5 years relevant laboratory experience, 2 of which must be in research.

Preferred: BS in Biochemistry, Chemistry, Biology, Microbiology, Genetics, or related discipline; six months to one (1) year of experience in a laboratory.

Indiana University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, ethnicity, religion, age, sex, sexual orientation or identity, national origin, disability status, or protected veteran status. This institution is also a provider of ADA services.